human prostate cancer cells c4 2b  (ATCC)

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and DU145). Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Concentration Assay

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Effects of flavonoids on mitochondrial membrane potential (MMP) in PC3 and DU145 prostate cancer cell lines. MMP was assessed using rhodamine 123 fluorescence intensity, expressed as relative fluorescence units (RFU), and normalized to the untreated control group (100%). Lower percentages indicate greater MMP depolarization. Cells were treated with two concentrations (50 µM and 100 µM) of each compound. Data are presented as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates. Statistical significance ( p < 0.05) of treatment effect compared to control (untreated cells) was determined using two-way ANOVA with Sidak's multiple comparisons test. *** p < 0.001.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Membrane, Fluorescence, Control

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: The representative dot plots display how the viable cells (Annexin V − /PI − ), early apoptotic cells (Annexin V + /PI − ), late apoptotic cells (Annexin V + /PI + ), and necrotic cells (Annexin V − /PI + ) were distributed after the 24-hours treatment. The control group was treated with the vehicle only. Effect of selected compounds (V3, V5, and V6) on apoptosis induction in PC3 and DU145 prostate cancer cells as assessed by Annexin V/Propidium Iodide.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Control

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Annexin V/PI analysis of apoptosis in PC3 and DU145 prostate cancer cells treated with flavone derivatives. DU145 cells were treated with 10 µM V3 (4′-hydroxy-3,5,6,7-tetramethoxyflavone), V5, or V6 for 24 h and stained with Annexin V-FITC/propidium iodide. Flow cytometric analysis quantified the percentage of viable, early apoptotic, and late apoptotic cells. Data were analyzed by two-way ANOVA with Sidak multiple comparisons test. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01; ns, not significant.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Staining

Journal: RSC Advances

Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

doi: 10.1039/d5ra09216g

Figure Lengend Snippet: Cell cycle profile following treatment with flavonoids V3. (A) A representative Kaluza cell cycle histogram of PI-stained PC3 and DU145 cells following 24 h-treatment of flavonoid V3 (10 µM) compared to untreated controls. (B–D) Progression of cell cycle phases assessed using ordinary one-way ANOVA with Dunnett's multiple comparisons test. Bars represent mean ± SEM of 2 independent biological replicates.

Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Staining

Journal: bioRxiv

Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer

doi: 10.64898/2026.03.05.709907

Figure Lengend Snippet: Engineering hPSMA-CARs with mbIL12 rescues CAR T cell functionality a Schema of hPSMA-CAR variants with mbIL12 expression. b Flow cytometric analysis of ET260-1 CAR and ET260-1(58) CAR with or without mbIL12 as detected by extracellular Fc and intracellular IL-12. c Tumor cell killing of hPSMA-CARs at an E:T of 1:4 over 72 hrs. d Expression of 4-1BB over 72 hrs on hPSMA-CARs. e Expression of CD25 on hPSMA-CARs after 72 hrs. f Secretion of IFNγ of hPSMA-CARs with mbIL12 after 72 hrs as determined by ELISA. g Tumor cell killing of hPSMA-CARs with mbIL12 and J591 against PC-3 PIP at E:T of 1:10 over 5 days at varying concentrations of anti-IFNγR1 blocking antibody and isotype control. Data are presented as mean values ± SEM. P values indicate differences between ET260-1-dCH2(28tm)BBz and ET260-1-dCH2(28tm)BBz/mbIL12 and between ET260-1-dCH2(28tm)BBz/mbIL12 and J591-dCH2(4tm)BBz using a two-tailed Student’s t-test.

Article Snippet: Human metastatic prostate cancer cell line PC-3 (ATCC CRL-1435) was cultured in RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Hyclone), and 1X antibiotic-antimycotic (AA, Gibco) (complete RPMI).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Blocking Assay, Control, Two Tailed Test

Journal: bioRxiv

Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer

doi: 10.64898/2026.03.05.709907

Figure Lengend Snippet: mbIL12-engineered hPSMA-CAR T cells demonstrate improved activity in a recursive tumor cell assay in vitro a Tumor cell killing of hPSMA-CARs over 8 days when co-cultured with PSMA-expressing cell lines at E:T of 1:20. b Fold expansion of hPSMA-CARs compared to J591-CAR after 8-day co-culture. c Production of IFNγ at 8 days as determined by ELISA. d Schema of rechallenge with PSMA-CARs co-cultured with PC-3 PSMA lo , PC-3 PSMA hi , or PC-3 PIP and rechallenged with tumor cells every 3 days. e PC-3 PSMA lo (left), PC-3 PSMA hi (center), or PC-3 PIP (right) cell counts were quantified by flow cytometry. b Fold expansion of T cells after each rechallenge were quantified by flow cytometry. c Production of IFNγ by T cells following each rechallenge as determined by ELISA. Data are presented as mean values ± SEM. P values indicate differences between ET260-1-dCH2(28tm)BBz and ET260-1-dCH2(28tm)BBz/mbIL12 and between ET260-1-dCH2(28tm)BBz/mbIL12 and J591-dCH2(4tm)BBz using a two-tailed Student’s t-test.

Article Snippet: Human metastatic prostate cancer cell line PC-3 (ATCC CRL-1435) was cultured in RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Hyclone), and 1X antibiotic-antimycotic (AA, Gibco) (complete RPMI).

Techniques: Activity Assay, In Vitro, Cell Culture, Expressing, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Two Tailed Test